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We have shown that T cell signalling is initiated upon ordered domain aggregation that can be triggered by cold stress and changes in the plasma membrane lipid composition. We have recently shown that the T cell receptor in resting T cells resides in ordered domains that are brought together upon receptor engagement.
We are now investigating what is triggering the formation of the ordered domains in more detail and how membrane order affects the molecular clustering that accompanies T cell signalling using techniques like super resolution microscopy and fluorescence correlation spectroscopy.
The localisation of the T cell receptor. The TCR is found in ordered plasma membrane domains that form where actin filaments are pinned to the plasma membrane. To scan the environment TCR-containing ordered domains at the tip of membrane protrusions positions the receptor at an ideal position. From Dinic et al., 2015
Dinic J, Ashrafzadeh P, Parmryd I. (2013) Actin filaments at the plasma membrane in live cells cause the formation of ordered lipid domains. Biochim. Biophys. Acta. 1828, 1102-1111.
Dinic J, Riehl A, Adler J, Parmryd I. (2015) The T cell receptor resides in small ordered plasma membrane domains that aggregate upon patching of the receptor. Sci. Rep. 5:10082.