Group members

The links below lead to University directory

Ingela Parmryd

Ongoing projects:
 

Ordered plasma membrane domains in T cell signalling

Membrane topography and diffusion in the plasma membrane

γ δ T cells in colon cancer, malaria infection and their activation

Colocalisation Analysis

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Membrane topography and diffusion in the plasma membrane

The cell surface is neither flat nor smooth but surface topography is ignored in current models of the plasma membrane. Using high resolution topographical maps of live cells, we and our collaborators have demonstrated that apparent topographical trapping is easily mistaken for elaborate membrane model features like hop diffusion and transient anchorage. Even binding could be the result of apparent topographical trapping when single particle tracks are interpreted in 2D although the molecules are moving in 3D. We are now together with collaborators at CBA at UU developing a method to analyse diffusion coefficients on non-flat surfaces.

High resolution hopping ion conductance microscopy image of part of a live FRSK cell. The figure shows that cell topography is an important factor when determining the diffusion coefficients of membrane molecules.
 

Selected publicaitons

Adler J, Novak P, Shevchuk AI, Korchev YE, Parmryd I. (2010) High resolution plasma membrane topography imaging for correct interpretation of single particle tracks. Nat. Methods 7, 170-171.

Parmryd I, Önfelt B. (2013) Consequences of membrane topography. FEBS J. 280, 2775-2784


For further information about this research group please contact
Docent Ingela Parmryd

Financial support

2016-2019

3.2 million SEK

Swedish
Research
Council

“The functional interplay between lipid packing and molecular clustering in T cell signalling”
 

2013-2016

3 million SEK

AFA
Insurance

“The expansion of highly potent tumour cell killing γ δ T cells from healthy individuals and cancer patients”



Collaborators

  • Dr Fredrik Bergholm (Royal Institute of Technology, Stockholm)
  • Professor Jonas Bergkvist (Department of Chemistry, Uppsala University)
  • Dr Helgi Birgisson (Department of Surgical Sciences, Uppsala University)
  • Professor Ingrid Faye (Department of Molecular Biosciences, Stockholm University)
  • Professor Yuri Korchev & Dr Andrew Shevshuk (Imperial College, London, UK)
  • Drs Ida-Maria Sintorn & Robin Strand(Centre for Image Analysis, Swedish University of Agricultural Sciences & Uppsala University)