Ingela Parmryd

Ongoing projects:
 

Ordered plasma membrane domains in T cell signalling

Membrane topography and diffusion in the plasma membrane

γ δ T cells in colon cancer, malaria infection and their activation

Colocalisation Analysis

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Membrane topography and diffusion in the plasma membrane

The cell surface is neither flat nor smooth but surface topography is ignored in current models of the plasma membrane. Using high resolution topographical maps of live cells, we and our collaborators have demonstrated that apparent topographical trapping is easily mistaken for elaborate membrane model features like hop diffusion and transient anchorage. Even binding could be the result of apparent topographical trapping when single particle tracks are interpreted in 2D although the molecules are moving in 3D. We are now together with collaborators at CBA at UU developing a method to analyse diffusion coefficients on non-flat surfaces.

High resolution hopping ion conductance microscopy image of part of a live FRSK cell. The figure shows that cell topography is an important factor when determining the diffusion coefficients of membrane molecules.
 

Selected publicaitons

Adler J, Novak P, Shevchuk AI, Korchev YE, Parmryd I. (2010) High resolution plasma membrane topography imaging for correct interpretation of single particle tracks. Nat. Methods 7, 170-171.

Parmryd I, Önfelt B. (2013) Consequences of membrane topography. FEBS J. 280, 2775-2784


For further information about this research group please contact
Docent Ingela Parmryd