Group members

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Ingela Parmryd

Ongoing projects:
 

Ordered plasma membrane domains in T cell signalling

Membrane topography and diffusion in the plasma membrane

γ δ T cells in colon cancer, malaria infection and their activation

Colocalisation Analysis

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Colocalisation Analysis

We develop image analysis software to get quantitative and objective answers to our questions. We have developed and patented the method RBNCC (replicate based noise corrected correlation) where image noise, which is unavoidable and leads to the underestimation of the underlying correlation, can be eliminated from correlation measurements. RBNCC forms the basis for our company No More Noise.

We have performed detailed studies on coefficients developed for use in colocalisation analyses revealing that several are not fit for their purpose. We advocate that coloocalisation analysis should be divided into the two subgroups co-occurrence and correlation and that only pixels where both fluorophores are present should be included in correlation analyses. We now investigate how deconvolution affects correlation analysis and how different intracellular distributions of molecules are represented by our division of colocalisation analysis into co-occurrence and correlation.

Selected publications

Adler, J, Parmryd, I. (2013) Colocalization analysis in fluorescence microscopy. Methods Mol. Biol. 931, 97-109

Adler J, Parmryd I. (2014) Quantifying Colocalisation: empty pixels, thresholding and the Hcoef. PLoS One 9:111983


For further information about this research group please contact
Docent Ingela Parmryd

Financial support

2016-2019

3.2 million SEK

Swedish
Research
Council

“The functional interplay between lipid packing and molecular clustering in T cell signalling”
 

2013-2016

3 million SEK

AFA
Insurance

“The expansion of highly potent tumour cell killing γ δ T cells from healthy individuals and cancer patients”



Collaborators

  • Dr Fredrik Bergholm (Royal Institute of Technology, Stockholm)
  • Professor Jonas Bergkvist (Department of Chemistry, Uppsala University)
  • Dr Helgi Birgisson (Department of Surgical Sciences, Uppsala University)
  • Professor Ingrid Faye (Department of Molecular Biosciences, Stockholm University)
  • Professor Yuri Korchev & Dr Andrew Shevshuk (Imperial College, London, UK)
  • Drs Ida-Maria Sintorn & Robin Strand(Centre for Image Analysis, Swedish University of Agricultural Sciences & Uppsala University)