Techniques seminar series

This seminar series is intended to provide a thorough coverage of experimental techniques including measurements and experimental data processing concepts. Presentations on new techniques, technical developments or discussion regarding to experimental problems/improvements are also welcome.

All interested are welcome to attend.

If you like to present a technique or you are interested in a particular technology/method, please contact My Quach (


Total internal reflection fluorescence imaging: Shedding light on cellular processes near the plasma membrane


Professor Anders Tengholm,
Department of Medical Cell Biology, Uppsala University
Tuesday, April 25th, 2017
BMC C2:305 , 14:15

Total internal reflection fluorescence (TIRF) microscopy, also known as evanescent wave microscopy, is a light microscopy technique, which enables selective excitation of fluorescent molecules in a very restricted volume of the specimen immediately adjacent to the coverslip. Examples will be given on some of the many applications in cell biology, including studies of cell-substrate contacts, intracellular signaling and vesicle trafficking.