Methods

Most of our studies are conducted in vivo in transgenic mice, which enable visualization of leukocyte-endothelial interactions in different organs (bright-field or high-speed confocal microscopy) in addition to registration of blood perfusion (Laser Doppler and Laser Speckle), bacterial clearance (non-invasive IVIS) or mucus thickness (microelectrodes) etcetera. Inflammation is induced either by administration of one or more specific chemokines (applied protein, or through plasmid DNA delivery), DSS colitis, allogeneic transplantation or bacterial infection. The models of hypoxia used are the recently developed model of transplantation of isolated pancreatic islets as well as the ischemic hind limb. Tissue restoration is followed in injured tissues or following induction of wounds. To assess the identity of the involved immune cells during the different situations, single cell suspensions are prepared and cells are analyzed for proteomics and transcriptomics.

Contact: Mia Phillipson