Olof Idevall-Hagren

Ongoing projects:

Organelle communication via membrane contacts sites (MCS)

Extended-Synaptotagmins generate ER-PM contacts

Optogenetic tool development and implementation

Plasma membrane PI(4,5)P2 regulate insulin secretion

Expand / collapse all projects


Plasma membrane PI(4,5)P2 regulate insulin secretion

PI(4,5)P2 is a rare lipid in the plasma membrane known as a master regulator with targets including components of receptor signaling, endo- and exocytosis and ion channels. In recent work, using optogenetic depletion and synthesis of PI(4,5)P2, we found this lipid to control voltage-dependent Ca2+ influx in ß-cells. Light-induced removal of PI(4,5)P2 acutely blocked Ca2+ increases induced by glucose, resulting in strong inhibition of insulin secretion (see Figure 4). We are currently investigating additional roles of this lipid in the regulation of ß-cell function.

Figure 4: Traces showing measurements of the cytosolic Ca2+ concentration in glucose-stimulated b-cells. In control cells (left), blue-light illumination did not alter the regular Ca2+ oscillations, whereas these were completely abolished in cells expressing a light-regulated PI(4,5)P2 phosphatase (right).


For further information about this research group please contact
Olof Idevall-Hagren