Mechanisms of regulated exocytosis
Exocytosis is fundamental to every cell and crucial to intracellular transport, protein sorting, and cell-to-cell communication. In endocrine cells and neurons, exocytosis leads to the release of hormones and transmitter molecules, and defects in this process often underlie diseases, such as type-2 diabetes and some forms of neurodegeneration. My lab is interested in the cellular mechanisms by which insulin and glucagon are released in the endocrine pancreas, and how disease affects these. Our work combines advanced light microscopy (TIRF, super-resolution and single molecule imaging), patch clamp electrophysiology, and genetic manipulation, which allows us to observe single secretory granules and even individual protein molecules in living cells.